For Peptides, Proteins and Biomolecules
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High Performance Columns and Guard Systems.
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Thomson introduces new reverse phase columns for the chromatography of peptides and proteins. They are characterized by high resolution, efficiency and peak symmetry. These products are based on an ultra clean silica. Our bonding procedure virtually eliminates the tailing characteristic of basic compounds. They are available with 100Å or 300Å pores in C8 or C18 phases. These unique materials exhibit the same selectivity independent of pore size. Both 3 and 5µm particle sizes are available Take advantage of the better resolution, for small molecules, afforded by the high surface area, 100Å packing, or look at larger molecules on the 300Å packing. Their identical selectivity allows you to maintain the same elution order from one product to the other. Chromatographing small proteins or peptides on a 300Å packing is conventional wisdom. But in many cases the higher surface area support would be the better choice. Choose, the C8 phase rather than C4. In most cases, it will effect a separation comparable to one done on C4 and C8 is more stable. |
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BIOAdvantageTM HL C18 combines high surface area with high carbon load. Both parameters are important when considering carbon content and its impact on your chromatography. Historically it has been difficult to achieve a high carbon loaded reverse phase packing that was also highly efficient and produced good peak shapes. BIOAdvantage HL does this. It's designed to give higher k’s (retention) allowing the chromatographer to achieve separations in less time with shorter columns that consume less solvent. Speed, less dilution (greater sensitivity) and excellent peak shapes, |
even for basic compounds, are the benefits of this type of column technology. The chromatogram of four parabens illustrates this. The HL column, packed with 5 µm particles, is intended to facilitate rapid separation of compounds with comparable resolution, speed and sensitivity to a 3 µm material. But with less back pressure and less potential to plug. How well this column works is illustrated also by the excellent resolution of Hodges peptides. BIOAdvantage HL exhibits almost baseline resolution for the very difficult to separate first pair. These are just some of the BIOAdvantageTM advantages. |
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BIOAdvantage Basic & CL areunique materials ideally suited for chormatographing basis comounds. This is illustrated by the chromatogram of pyridine and phenol in water/acetronitrile unmodified by TFA or other enhancing additive. Other columns, thought to be the standard of the industry, make |
these compounds tail badly and require suitable modifier to "shield" active sites on the solute or packing surface. This is illustrated by the separation of phenol and pyridine on a BIOAdvantage column when comared to another "base deactivated" column. |
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