Base Deactivated Columns for Polar Molecules
 BioAdvantage Basic™
  • For polar molecules
  • High efficiencies
  • Excellent peak shapes,
    without modifiers

Polar molecules are a problem for most silica based columns. Their polar surface interacts with the solute, sometimes proving critical to the separation, but in the case of polar amines, often causing them to tail badly.

This can be overcome with ionic modifiers such as buffers and TFA which either "tie" up the solute to prevent this interaction or shield the residual silanols from the solute.

 
BioAdvantage Basic is designed to eliminate the need for these modifers.

This is illustrated by the chromatogram at the left separating pyridine and phenol with out any mobile phase "helpers".


Separation of
Pyridine and Phenol
40% acetonitile/water, unbuffered.
Peak 1: Pyridine, Peak 2: Phenol

This is illustrated in practical terms by the peptide chromatogram below. Note the low amounts used and the change in selectivity when acetic acid is used rather than TFA.

Separation of peptides with 0.1% acetic acid
on BioAdvantage Basic.
Separation of peptides with 0.1% TFA
on BioAdvantage Basic.


BioAdvantage Basic performs better than
major brand's deactivated packing.
Comparison of BioAdvantage Basic (bottom chromatogram) to another popular base deactivated reverse phase column (top). Notice the band broadening and reversal of elution order on the competitive column.

The mobile phase is 20%MeOH/Water. In the top chromatogram, phenol elutes before pyridine. Tailing is bad for both molecules. On BioAdvantage Basic, peaks are sharp and pyridine elutes first.

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