Column Type:
BioAdvantage
HL C18, 4.6 x 150 mm
Mobile
Phase:
A: 0.1% TFA in Water
B: 0.1% TFA in Acetonitrile
Gradient:
Linear gradient, 1 ml/min, 0 to 30% solvent B in 30 minutes.
Detection:
UV 210 nm
Discussion:
The Canadian corporation , SPI, produces a well characterized, challenging
peptide mixture that is very useful for quantitying HPLC column performance
for peptides. The difficult to separate first pair of decapeptides
are near base line resolved on Advantage HL C18 columns.
Peak
Identities:
1. H2N-RGAGGLGLGK-amide
2. AC-RGGGGLGLGK-amide
3. AC-RGAGGLGLGK-amide
4. AC-RGVGGLGLGK-amide
5. AC-RGWGLGLGK-amide
