Column Type:
Advantage 100 C18, 5 µm, 4.6 x 100 mm

Mobile Phase:
A: 0.1M TEAA, pH 7.0 B: Acetonitrile

Gradient:
Linear gradient, 1 mi/min from 8-15% in 24 mins

Detection:
UV, 260 nm

Disscussion: Trityl-off chromatography is a routine method used for purification and purity determination of synthetic oligonucleotides

Peak Identies:

1. Benzamide
2. 5' TGG CAA GCC ACG TTT GGT 3'