Column Type:
BioAdvantage HL, 5µ, 4.6 x 100 mm

Mobile Phase:
70% Acetonitrile in water

Conditions:
Isocratic, 1 ml/min

Detection:
UV, 254 nm

Discussion:
Fast analysis, symmetrical peaks and baseline resolution are characteristics of paraben analysis on short, high carbon load C18 column.

 Peak Identities:
   I. Methyl paraben
  2. Ethyl paraben
  3. Propy] paraben
  4. Butyl paraben