Column Type: Advantage 100 C18, 4.6 x 250 mm

Mobile Phase: A: 50 mM phosphoric acid, pH  2.8
B: 60% Acetonitrile / 40% A

Gradient:
1 mi/min
0 min 0%B
5 min 0%B
120 min 40%B
130 min 60%B

Instrument:
PerSeptive Biosystems Integral,  215 nm