SEDEX ELS DETECTOR APPLICATIONS

Sample: Phospholipids

HPLC Conditions Column: Lichrospher Si 60-5µm (4x125mm) Eluent : A: CHCl3/MeOH/H20/NH3(30%) (60:34:5.5:0.5) B: CHCl3/MeOH/NH3(30%) (80:19.5:0.5) 0 to100%(A) in 14min / 100%(A) in 9 min. Flow rate: 1ml/min Nebulization gas: Air Temperature 50°C   1. Nonpolar lipids  2. CR: cerebroside- fatty acid not hydroxylated  3. CROH: cerebroside-fatty acid hydroxylated  4. PE: phosphatidyl ethanolamine  5. PI: phosphatidyl inositol  6. PS: phosphatidyl serine  7 PC: phosphatidyl choline  8. PA: phosphatidyl acid  9. Sph: sphingomyelin 10. LPC: lysophosphatidyl choline

Detector Sensitivity
Sample A: Phosphatidyl choline, 100ng B: Phosphatidyl ethanolamine, 100 ng HPLC Conditions Column : Lichrospher si 60 - 5 µm 125 mm x 4 mm Injected volume : 10 µl Eluent : A : CHCl3/MeOH/H20/NH3 30% (60/34/5.5/0.5) B: CHCl3/MeOH/NH3 30% (80/19.5/0.5) Flow rate : 1ml/min Pressure : 2.2 bars Gas: Air Temperature : 50°C