SEDEX ELS DETECTOR APPLICATIONS

Sample: Sugars
	HPLC Conditions Column: Lichrospher 100 Diol-10µm (7x250mm) Eluent: A: Acetonitrile / B: Water (10 to 50% (A) in 20min. 50 to 70%(A) in 5min.) Flow rate: 1ml/min Nebulization gas: Air Temperature: 45°C 1. Fructose (200ppm) 2. Glucose 3. Sucrose 4. Lactose 5. Raffinose 6. Dextrans

High Sensitivity

HPLC Conditions Column: Asahipak - NH2. 250 x 4.6 mm Injected volume: 20 µl - 2ppm Eluent: A: Acetonitrile / B: Water Flow rate: 1ml/min Pressure: 2 bars Gas: Air Temperature: 50°C Peaks F Fructose G Glucose L Lactose S Sucrose A Aaffinose St Stachyose Gradient B from 25% to 50% in 15 min