Polymer-Based Columns (RSpak)
Partition and adsorption chromatography is the most widely used analytical method
in HPLC analysis. Shodex polymer-based columns for partition and adsorption chromatography
(reversed and normal phase) is superior to silica-based media in range of eluent
selection, lot reproducibility and durability. In addition to standard columns
with ID 4.6-8.0mm, we provide micro columns with ID 300micron-800micron, semi-micro
columns with ID 1.0mm-2.0mm and preparative columns with ID 10mm and above.
| RP18-415 |
Suitable for protein and peptide analysis because of the large
pore size (450 angstrom) |
| RP18-613,413 |
High performance version (high theoretical plate number) of
DS-613 and DS-413. |
| DS-613,413 |
Because they are packed with low polarity polymers (styrene
divinyl benzene), these are suitable for analysis by reversed phase of highly
hydrophilic substances which cannot be well-retained by ODS columns. |
| DE Series |
These columns have the widest application among
the RSpak. Since they are packed with polymer-based material that have almost
equal polarity as silica-based material bonded with C-18 groups, chromatography
similar to ODS columns can be obtained. They have a wide application and
can be used with eluent conditions that are not compatible with standard
ODS columns, such as "can be used in analysis with 100% water" and "can
be used in analysis and cleaning with alkali eluent". Separation characteristics
of the DE series are slightly different from those of the Asahipak ODP series
because of the difference in packing material. |
| DM-614 |
Suitable for analysis of amino acids and polypeptides. |
| DC-613 |
Suitable for analysis of saccharides in normal phase. |
| NN-814,614,414 |
Multimode column
with reversed phases and cation exchange.
These columns incorporate a trace amount of sulfo group in the packing
material of the reversed phase columns. Separation of neutral substances
is by reversed phase mode, of basic substances by reversed phase and ion
exchange mode and of acidic substances by ion exclusion and reversed phase
mode. They are therefore suitable for analysis of complex samples that have
a mixture of neutral substances and ionic substances. |
JJ-50
(old name) SS-50 |
Multimode
column with reversed phases and anion exchange.
These columns incorporate a trace amount of quaternary ammonia group in
the packing material of the reversed phase columns. Separation of neutral
substances is by reversed phase mode, of basic substances by ion exclusion
and reversed phase mode and of acidic substances by reversed phase and
ion exchange mode. They are therefore suitable for analysis of complex
samples that have a mixture of neutral substances and ionic substances.
|
RSpak RP18-415, 613, 413
(Standard columns)
| Product name |
Plate number
(TPN/column) |
Particle size
(micron) |
Size(mm)
ID x Length |
RSpak RP18-415
RSpak RP18-613
RSpak RP18-413
RSpak RP18-G |
> 5,000
> 13,000
> 11,000
Guard column |
6
3.5
3.5
6 |
4.6 x 150
6.0 x 150
4.6 x 150
4.6 x 10
|
| Product name |
Max. usable
pressure
(MPa) |
Max. usable
flow rate
(mL/min) |
Usable
temp. range
(deg-C) |
Usable
pH range |
Usable salt
conc. |
In-column
solvent |
RP18-415
RP18-613
RP18-413
RP18-G |
22
22
22
- |
1.5
1.5
1.0
- |
RT to 50
RT to 50
RT to 50
RT to 50 |
1 to 13
1 to 13
1 to 13
1 to 13 |
< 0.5M
< 0.5M
< 0.5M
< 0.5M
|
CH3CN/H2O=80/20
CH3CN/THF/H2O=30/30/40
CH3CN/THF/H2O=30/30/40
CH3CN/THF/H2O=30/30/40
|
When you do not use a column, please keep it between pH 2 to 12.
In the case of keeping a column for a long time, please replace with the shipping
solvent in the column.
Eluent conditions:
| Product name |
Toluene
Chloroform etc. |
Methanol |
Acetonitrile |
THF |
Water solusiton |
RP18-415
RP18-613
RP18-413 |
0 to 50%
0 to 50%
0 to 50%
|
0 to 100%
30 to 100%
30 to 100%
|
0 to 100%
10 to 100%
10 to 100%
|
0 to 50%
5 to 100%
5 to 100%
|
0 to 100%
cannot use 100% Water solusiton
cannot use 100% Water solusiton
|
RSpak DS-613, 413, 2013, 5013
(Standard columns)
| Product name |
Plate number
(TPN/column) |
Particle size
(micron) |
Size(mm)
ID x Length |
RSpak DS-613
RSpak DS-413
RSpak DS-G |
> 6,500
> 11,000
Guard column |
6
3.5
10 |
6.0 x 150
4.6 x 150
4.6 x 10
|
| Product name |
Max. usable
pressure
(MPa) |
Max. usable
flow rate
(mL/min) |
Usable
temp. range
(deg-C) |
Usable
pH range |
>Usable
salt conc. |
In-column
solvent |
DS-613
DS-413
DS-G |
10
20
- |
3.0
1.0
- |
RT to 80
20 to 50
RT to 80 |
2 to 12
*1 to 13
*1 to 13 |
< 0.5M
< 0.5M
< 0.5M |
CH3CN/THF/H2O=40/30/30
CH3CN/THF/H2O=30/30/40
-
|
When you do not use a column, please keep it between pH 2 to 12.
In the case of keeping a column for a long time, please replace with the shipping
solvent in the column.
Eluent conditions: Please refer to
Eluent conditions
for RSpak D sereies.
RSpak DE Series
(Standard columns)
| Product name |
Plate number
(TPN/column) |
Particle size
(micron) |
Size(mm)
ID x Length |
Applications |
RSpak DE-613
RSpak DE-413
RSpak DE-413L
RSpak DE-413S
RSpak DE-G
RSpak DE-213
RSpak DE-SG |
> 7,000
> 11,000
> 17,000
> 3,000
Guard column
> 8,000
Guard column
|
6
4
4
4
10
4
4 |
6.0 x 150
4.6 x 150
4.6 x 250
4.6 x 50
4.6 x 10
2.0 x 150
2.0 x 10
|
Column-switching
LC/MS analyses
|
| Product name |
Max. usable
pressure
(MPa) |
Max. usable
flow rate
(mL/min) |
Usable
temp. range
(deg-C) |
Usable
pH range |
>Usable
salt conc. |
In-column
solvent |
DE-613
DE-413
DE-413L
DE-413S
DE-G
DE-213
DE-SG |
7
15
18
-
-
12
- |
3.0
1.2
1.2
-
-
0.4
- |
RT to 70
RT to 60
RT to 60
RT to 60
RT to 70
RT to 60
RT to 60 |
2 to 12
2 to 12
2 to 12
2 to 12
2 to 12
2 to 11
2 to 11 |
< 0.5M
< 0.5M
< 0.5M
< 0.5M
< 0.5M
< 0.5M
< 0.5M
|
H2O
CH3CN/H2O=50/50
CH3CN/H2O=50/50
CH3CN/H2O=50/50
H2O
CH3CN/H2O=50/50
-
|
Eluent conditions: Please refer to
Eluent conditions for RSpak D sereies.
(Micro column and Semi-micro columns)(Remark)
(Preparative Columns)(Remark)
| Product name |
Particle size
(micron) |
Size(mm)
ID x Length |
Standard
column |
RSpak DE-2013
RSpak DE-LG |
12
12 |
20.0 x 300
8.0 x 50
|
DE-613
Guard column
|
RSpak DE-5013
RSpak DE-LLG |
12
12 |
50.0 x 500
20.0 x 100
|
DE-613
Guard column
|
RSpak DM-614, 2013, 5013
(Standard columns)
| Product name |
Plate number
(TPN/column) |
Particle size
(micron) |
Size(mm)
ID x Length |
RSpak DM-614
RSpak DM-G |
> 4,500
Guard column |
10
12 |
6.0 x 150
4.6 x 10
|
| Product name |
Max. usable
pressure
(MPa) |
Max. usable
flow rate
(mL/min) |
Usable
temp. range
(deg-C) |
Usable
pH range |
Usable
salt conc. |
In-column
solvent |
DM-614
DM-G |
2
- |
2.0
- |
RT to 60
- |
2 to 10
- |
< 0.5M
- |
0.005M H3PO4
-
|
Eluent conditions: Please refer to
Eluent conditions
for RSpak D sereies.
(Preparative Columns)(Remark)
| Product name |
Particle size
(micron) |
Size(mm)
ID x Length |
Standard
column |
RSpak DM-2014
RSpak DM-LG |
12
12 |
20.0 x 300
8.0 x 50
|
DM-614
Guard column
|
RSpak DM-5014
RSpak DM-LLG |
12
12 |
50.0 x 500
20.0 x 100
|
DM-614
Guard column
|
RSpak DC-613, 2013, 5013
(Standard columns)
| Product name |
Plate number
(TPN/column) |
Particle size
(micron) |
Size(mm)
ID x Length |
RSpak DC-613
RSpak DC-G |
> 5,500
Guard column |
6
10 |
6.0 x 150
4.6 x 10
|
Product
name |
Max. usable
pressure
(MPa) |
Max. usable
flow rate
(mL/min) |
>Usable
temp. range
(deg-C) |
Usable
pH range |
Usable
salt conc. |
In-column
solvent |
DC-613
DC-G |
5
- |
3.0
- |
40 to 85
40 to 85 |
2 to 14
2 to 14 |
< 0.5M
< 0.5M |
CH3CN/H2O=70/30
CH3CN/H2O=70/30
|
Eluent conditions: Please refer to
Eluent conditions for RSpak D sereies.
(Preparative Columns)(Remark)
| Product name |
Particle size
(micron) |
Size(mm)
ID x Length |
Standard
column |
RSpak DC-2013
RSpak DC-LG |
10
10 |
20.0 x 300
8.0 x 50
|
DC-613
Guard column
|
RSpak DC-5013
RSpak DC-LLG |
10
10 |
50.0 x 500
20.0 x 100
|
DC-613
Guard column
|
RSpak NN Series
(Standard columns)
| Product name |
Plate number
(TPN/column) |
Particle size
(micron) |
Size(mm)
ID x Length |
RSpak NN-814
RSpak NN-G
RSpak NN-614
RSpak NN-414 |
> 9,000
Guard column
> 4,000
> 6,000 |
10
10
10
10 |
8.0 x 250
6.0 x 50
6.0 x 150
4.6 x 150
|
| Product name |
Max. usable
pressure
(MPa) |
Max. usable
flow rate
(mL/min) |
Usable
temp. range
(deg-C) |
Usable
pH range |
Usable
salt conc. |
In-column
solvent |
NN-814
NN-G
NN-614
NN-414 |
2.0
-
1.5
1.2 |
1.5
-
1.0
0.5 |
10 to 50
10 to 50
10 to 50
10 to 50 |
2 to 12
2 to 12
2 to 12
2 to 12 |
< 0.5M
< 0.5M
< 0.5M
< 0.5M |
0.1M NaH2PO4
0.1M NaH2PO4
0.1M NaH2PO4
0.1M NaH2PO4
|
Eluent conditions: Please refer to
Eluent conditions
for RSpak NN sereies.
(Micro column and Semi-micro columns)(Remark)
RSpak JJ-50
(Standard columns)
| Product name |
Plate number
(TPN/column) |
Particle size
(micron) |
Size(mm)
ID x Length |
RSpak JJ-50 4D
RSpak JJ-50 2D |
> 4,500
> 3,500 |
5
5 |
4.6 x 150
2.0 x 150
|
| Product name |
Max. usable
pressure
(MPa) |
Max. usable
flow rate
(mL/min) |
Usable
temp. range
(deg-C) |
Usable
pH range |
Usable
salt conc. |
In-column
solvent |
JJ-50
4D
JJ-50 2D |
9.0
9.0 |
1.2
0.3 |
10 to 60
10 to 60 |
2 to 10
2 to 10 |
< 0.1M
< 0.1M |
0.005M H3PO4
0.005M H3PO4
|
