Polymer-Based Columns (Asahipak)

Partition and adsorption chromatography is the most widely used analytical method in HPLC analysis. Shodex polymer-based columns for partition and adsorption chromatography (reversed and normal phase) is superior to silica-based media in range of eluent selection, lot reproducibility and durability. In addition to standard columns with ID 4.6-6.0mm, we provide micro columns with ID 300micron-800micron, semi-micro columns with ID 1.0mm-2.0mm and preparative columns with ID 10mm and above.

ODP
C8P
C4P		 Since the pore size of packing material for Asahipak ODP, C8P and C4P series is large, only one type of column is needed for the analysis of high molecular weight substances such as proteins to medium/low molecular weight substances such as peptides and amino acids. Hydrophobic interaction becomes weaker in the following order: ODP (octadecyl group), C8P (octyl group) and C4P (butyl group). Characteristics similar to the RSpak DE series can be found, when used in analysis with 100% water, when used with alkali eluent and when used in with low salt concentration.
ODP-40 High performance version of the ODP-50 series. ODP-50G 4A of the ODP-50 series must be used a the guard column.
NH2P These are polymer-based amino columns that incorporate an amino group in the polyvinyl alcohol based gel. They are suitable for analysis of saccharides in normal phase like silica-based amino columns (p.23, Fig. 7-7). They are superior to silica-based amino columns in terms of reproducibility and durability because of their stability in a wide range of pH.

ODP,D8P,D4P
(Standard Columns)
Column type Plate number
(TPN/column)		 Particle
size
(micron)		 Size(mm)
ID x Length		 Carbon
(%)
Usable
pressure
(MPa)
 Usual
flow rate
(mL/min)		 Max.
flow rate
(mL/min)
Asahipak ODP-40 4D
Asahipak ODP-40 4E
> 11,000
> 17,000
 4
4
4.6 x 150
4.6 x 250
 17
17		 > 9
> 13
 0.3 to 0.6
0.3 to 0.6		 0.8
0.8
Asahipak ODP-50 6D
Asahipak ODP-50 6E
Asahipak ODP-50G 6A
> 9,000
> 14,000
Guard column
 5
5
5
6.0 x 150
6.0 x 250
6.0 x 10
 17
17
17		 > 15
> 15
-
 0.5 to 1.0
0.5 to 1.0
-		 2.5
2.5
-
Asahipak ODP-50 4B
Asahipak ODP-50 4D
Asahipak ODP-50 4E
Asahipak ODP-50G 4A
> 2,500
> 9,000
> 14,000
Guard column
 5
5
5
5
4.6 x 50
4.6 x 150
4.6 x 250
4.6 x 10
 17
17
17
17		 > 5
> 9
> 15
-		 0.4 to 0.6
0.3 to 0.6
0.5 to 0.8
-		 1.5
0.8
1.5
-
Asahipak ODP-50 2D
Asahipak ODP-50G 2A
> 5,000
Guard column
 5
5
2.0 x 150
2.0 x 10
 17
17		 > 12
-		 -
-		 0.4
-
Asahipak C8P-50 4D
Asahipak C8P-50 4E
Asahipak C8P-50G 4A
> 7,000
> 11,000
Guard column
 5
5
5
4.6 x 150
4.6 x 250
4.6 x 10
 10
10
10		 > 15
> 15
-		 0.5 to 0.8
0.5 to 0.8
-		 1.0
1.0
-
Asahipak C4P-50 4D
Asahipak C4P-50 4E
Asahipak C4P-50G 4A
> 6,000
> 9,000
Guard column
 5
5
5
4.6 x 150
4.6 x 250
4.6 x 10
 6
6
6		 > 15
> 15
-		 0.5 to 0.8
0.5 to 0.8
-		 1.0
1.0
-
For all column types:
Max. pressure: ODP-40 4D 9MPa, other column 15MPa
Usable temperature: 4 to 60 deg-C
Usable pH range: 2 to 13
Eluent conditions: Please refer to Eluent conditions for Asahipak (ODP, C8P, C4P, NH2P).

(Micro column and Semi-micro columns)(Remark)
Column
type		 Size(mm)
ID x Length		 Standard
column
ODP50-2E
ODP50-2D
ODP50-2B
ODP50-2T
2.0 x 250
2.0 x 150
2.0 x 50
2.0 x 35
ODP-50
ODP50-1E
ODP50-1D
ODP50-1B
ODP50-1T
1.0 x 250
1.0 x 150
1.0 x 50
1.0 x 35
ODP-50
ODP50-M8D
ODP50-M8B
ODP50-M8T
0.8 x 150
0.8 x 50
0.8 x 35
ODP-50
ODP50-M5D
ODP50-M5B
ODP50-M5T
0.5 x 150
0.5 x 50
0.5 x 35
ODP-50
ODP50-M3D
ODP50-M3B
ODP50-M3T
0.3 x 150
0.3 x 50
0.3 x 35
ODP-50

(Preparative Columns)(Remark)
Column type Particle
size
(micron)		 Size(mm)
ID x Length		 Standard
column
Asahipak ODP-50 10E
Asahipak ODP-90 20F
Asahipak ODP-130 28F
Asahipak ODP-130G 7B
Asahipak ODP-130G 20C
 5
9
13
13
13
10.0 x 250
20.0 x 300
28.0 x 300
7.5 x 50
20.0 x 100
ODP-50
ODP-50
ODP-50
Guard column
Guard column
Asahipak C8P-50 10E
Asahipak C8P-50G 7B
Asahipak C4P-50 10E
Asahipak C4P-50G 7B
 5
5
5
5
10.0 x 250
7.5 x 50
10.0 x 250
7.5 x 50
C8P-50
Guard column
C4P-50
Guard column

NH2P
(Standard Columns)
Column type Plate number
(TPN/column)		 Particle
size
(micron)		 Size(mm)
ID x Length
Usable
pressure
(MPa)
 Usual
flow rate
(mL/min)		 Max.
flow rate
(mL/min)
Asahipak NH2P-50 4D
Asahipak NH2P-50 4E
Asahipak NH2P-50G 4A
>5,500
>7,500
Guard column
 5
5
5
4.6 x 150
4.6 x 250
4.6 x 10
 > 15
> 9
- 		0.5 to 1.0
0.4 to 0.8
-		 1.5
1.5
-
Asahipak NH2P-50 2D
Asahipak NH2P-50G 2A
>3,500
Guard column
 5
5
2.0 x 150
2.0 x 10
 > 12
-
 0.2
-		 0.4
-
Asahipak NH2P-LF
Line filter
 5
8.0 x 75
 - - -
For all column types:
Usable temperature: 4 to 50 deg-C
Usable pH range: 2 to 13
Eluent conditions: Please refer to Eluent conditions for Asahipak (ODP, C8P, C4P, NH2P).

(Micro column and Semi-micro columns)(Remark)
Column
type		 Size(mm)
ID x Length		 Standard
column
NH2P50-2E
NH2P50-2D
NH2P50-2B
NH2P50-2T
2.0 x 250
2.0 x 150
2.0 x 50
2.0 x 35
NH2P-50
NH2P50-1E
NH2P50-1D
NH2P50-1B
NH2P50-1T
1.0 x 250
1.0 x 150
1.0 x 50
1.0 x 35
NH2P-50
NH2P50-M8D
NH2P50-M8B
NH2P50-M8T
0.8 x 150
0.8 x 50
0.8 x 35
NH2P-50
NH2P50-M5D
NH2P50-M5B
NH2P50-M5T
0.5 x 150
0.5 x 50
0.5 x 35
NH2P-50
NH2P50-M3D
NH2P50-M3B
NH2P50-M3T
0.3 x 150
0.3 x 50
0.3 x 35
NH2P-50

(Preparative Columns)(Remark)
Column type Particle size
(micron)		 Size(mm)
ID x Length		 Standard
column
Asahipak NH2P-90 10E
Asahipak NH2P-90 20F
Asahipak NH2P-130 28F
Asahipak NH2P-130G 7B		 9
9
13
13
10.0 x 250
20.0 x 300
28.0 x 300
7.5 x 50
NH2P-50
NH2P-50
NH2P-50
Guard column
Announcement: Change in ID of Asahipak Series Columns

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