Shodex: Packed Columns for HPLC Analysis

Features of Non-Porous Gel

Non-porous gel has high mechanical strength, which enables the separation at high flow rates for fast analysis.
The differences between porous and non-porous gel can be summarized as follows:
1) Max. working flow rate: DEAE-825 and SP-825 ( packed with porous gel ) : 1.5mL/min. DEAE-420N and SP-420N ( pack with non-porous gel ) : 2.0mL/min.
2)With the non-porous gel, the sample does not diffuse inside the pores of the packing material and the salt concentration of eluent equilibrates quickly, therefore sharp peaks can be obtained. However the maximum sample load is lower:
DEAE-825 and SP-825 ( packed with porous gel ) : 1,000micro-g/column DEAE-420N and SP-420N ( pack with non-porous gel ) : 20micro-g/column
3) In conclusion, columns packed with non-porous gel is suited for fast analysis of proteins and peptides, especially for the analysis for tracing the amount of samples.