Shodex: Packed Columns for HPLC Analysis

Affinity Chromatography Columns

Affinity chromatography is a well-known method for separating and purifying biological materials, based on their biochemical affinity. The Shodex AFpak series columns use rigid polymeric gel substrates with covalently attached ligands. In addition to standard columns with ID 4.6-8.0mm, we provide micro columns with ID 300micron-800micron, semi-micro columns with ID 1.0mm-2.0mm and preparative columns with ID 10mm and above. If a special column you need is not found in the following list, please contact your nearest representative office.

The columns have the following features:
1) Rigid polymeric media enable high-performance analysis.
2) Chemically stable bonding groups (spacers) between ligands and substrates minimize detachment of ligands, ensuring highly reproducible analysis. Up to hundreds of runs are reproducible.

Product name	Ligand	Applications
AFpak AAB-894 AFpak AAF-894 AFpak AAM-894 AFpak AAP-894 AFpak AAV-894 AFpak ABA-894 AFpak ABT-894 AFpak ACA-894 AFpak ACB-894 AFpak ADS-894	Aminobenzamidine Acrifla (AMP) Aprotinin Avidin Bovine serum albumin (BSA) Biotin Concanavalin A (ConA) Cibacron Blue Dextran sulfate	Serine protease RNA, DNA, Vitamines NAD, ATP dependent enzymes Serine proteases Biotin derivatives Chiral Separation(amino acid derivatives) Avidin Glycoprotein, polysaccharides Albumin, NDA dependent enzymes Lipoproteins, blood coagulation factors
AFpak AED-894 AFpak AGA-894 AFpak AGE-894 AFpak AGT-894 AFpak AHR-894 AFpak AIA-894 AFpak ALC-894 AFpak ALS-894 AFpak ANA-894 AFpak AOV-894	Ethylenediamine diacetic acid N-acetyl-glucosamine Gelatin Glutathione Heparin Iminodiacetic acid Lentil lectin Lysine (LCA) NAD Ovomucoid	Nucleic acids, serum proteins Lectins, carbohydrate metabolizing enzymes Fibronectin Enzymes related to glutathione Lipoproteins, blood coagulation factors Interferon, serum proteins Glycoproteins, polysaccharides Plasminogen, plasminogen activator, RNA, DNA NAD-Dependent dehydrogen Trypsin-like protease
AFpak APA-894 AFpak APB-894 AFpak APD-894 AFpak APE-894 AFpak APG-894 AFpak APH-894 AFpak APR-894 AFpak ARC-894 AFpak AST-894	Protein A Aminophenyl boronic acid Procion red Phosphorylethanolamine Protein G Phenyl alanine Proteamine RCA-1 (RCA-I) Soybean trypsin inhibitor	Human IgG, immune complexes Nucleic acids and cathecholamines NAD, NADP, interferon enzymes C-reactive protein, enzymes IgG immune complex Subtilisin Carlsberg IgM Glycoproteins, polysaccharides Trypsin-like proteases
AFpak AWG-894	Wheat germ agglutinin (WGA)	Glycoproteins, polysaccharides
(Enzyme-substrate immobilized column)
AFpak ACH-494 AFpak AGO-494	Choline oxydase, acetylcholine esterase Glucose oxydase	Choline, acetylcholine Glucose

(Standard columns)

Product name	Ligand load /gel(g)	Capacity	Particle size (micron)	Size(mm) ID x Length
AAB-894 AAF-894 AAM-894 AAP-894 AAV-894 ABA-894 ABT-894 ACA-894 ACB-894 ADS-894	100micro-mol 10micro-mol 10micro-mol 5mg 5mg 20mg - 15mg 40micro-mol 30mg	- ATP Na 1.8mg/golumn Lactic dehydronase 1.5mg/g Trypsin 3mg/g Biotin 8 micro-g/column - Avidin 9mg/g - BSA 20mg/column LDL 5mg/g	18 18 18 18 18 6 18 18 18 18	8.0 x 50 8.0 x 50 8.0 x 50 8.0 x 50 8.0 x 50 8.0 x 50 8.0 x 50 8.0 x 50 8.0 x 50 8.0 x 50
AED-894 AGA-894 AGE-894 AGT-894 AHR-894 AIA-894 ALC-894 ALS-894 ANA-894 AOV-894	30micro-mol - 30mg 50micro-mol 5mg 70micro-mol 6-7mg - 10micro-mol 10mg	- Lysozyme 0.6mg Fibronectin 150mg/g - Lysozyme 4mg/column BSA 70mg/column - Plasminogen 54micro-g/g Lactic dehydronase 1.4mg/g Trypin 100mg/g	18 18 18 18 18 18 18 18 18 18	8.0 x 50 8.0 x 50 8.0 x 50 8.0 x 50 8.0 x 50 8.0 x 50 8.0 x 50 8.0 x 50 8.0 x 50 8.0 x 50
APA-894 APB-894 APD-894 APE-894 APG-894 APH-894 APR-894 ARC-894 AST-894	4mg 800micro-mol 40-60micro-mol - 4-5mg 50micro-mol 4-5mg 20mg 20mg	IgG Human 20mg/g Sorbitol 0.2mg/column BSA 72mg/g C-reactive protein 0.91mg/g IgG Humnan 10mg/g Subtilisin Carlsberg 9mg/g IgM Human 1.9mg/column - Trypin 100mg/g	18 10 18 18 18 18 18 18 18	8.0 x 50 8.0 x 50 8.0 x 50 8.0 x 50 8.0 x 50 8.0 x 50 8.0 x 50 8.0 x 50 8.0 x 50
AWG-894	14mg	-	18	8.0 x 50
(Enzyme-substrate immobilized column)
ACH-494 AGO-494	- -	- -	18 18	4.6 x 10 4.6 x 10

(Micro column and Semi-micro columns)(Remark)

Product name	Size(mm) ID x Length
AAB-2B AAF-2B AAM-2B AAP-2B AAV-2B ABA-2B ABT-2B ACA-2B ACB-2B ADS-2B AED-2B AGA-2B AGE-2B AGT-2B AHR-2B AIA-2B ALC-2B ALS-2B ANA-2B AOV-2B APA-2B APB-2B APD-2B APE-2B APG-2B APH-2B APR-2B ARC-2B AST-2B AWG-2B	2.0 x 50 2.0 x 50 2.0 x 50 2.0 x 50 2.0 x 50 2.0 x 50 2.0 x 50 2.0 x 50 2.0 x 50 2.0 x 50 2.0 x 50 2.0 x 50 2.0 x 50 2.0 x 50 2.0 x 50 2.0 x 50 2.0 x 50 2.0 x 50 2.0 x 50 2.0 x 50 2.0 x 50 2.0 x 50 2.0 x 50 2.0 x 50 2.0 x 50 2.0 x 50 2.0 x 50 2.0 x 50 2.0 x 50 2.0 x 50

Product name	Size(mm) ID x Length
AAB-2T AAF-2T AAM-2T AAP-2T AAV-2T ABA-2T ABT-2T ACA-2T ACB-2T ADS-2T AED-2T AGA-2T AGE-2T AGT-2T AHR-2T AIA-2T ALC-2T ALS-2T ANA-2T AOV-2T APA-2T APB-2T APD-2T APE-2T APG-2T APH-2T APR-2T ARC-2T AST-2T AWG-2T	2.0 x 35 2.0 x 35 2.0 x 35 2.0 x 35 2.0 x 35 2.0 x 35 2.0 x 35 2.0 x 35 2.0 x 35 2.0 x 35 2.0 x 35 2.0 x 35 2.0 x 35 2.0 x 35 2.0 x 35 2.0 x 35 2.0 x 35 2.0 x 35 2.0 x 35 2.0 x 35 2.0 x 35 2.0 x 35 2.0 x 35 2.0 x 35 2.0 x 35 2.0 x 35 2.0 x 35 2.0 x 35 2.0 x 35 2.0 x 35

Product name	Size(mm) ID x Length
AAB-1B AAF-1B AAM-1B AAP-1B AAV-1B ABA-1B ABT-1B ACA-1B ACB-1B ADS-1B AED-1B AGA-1B AGE-1B AGT-1B AHR-1B AIA-1B ALC-1B ALS-1B ANA-1B AOV-1B APA-1B APB-1B APD-1B APE-1B APG-1B APH-1B APR-1B ARC-1B AST-1B AWG-1B	1.0 x 50 1.0 x 50 1.0 x 50 1.0 x 50 1.0 x 50 1.0 x 50 1.0 x 50 1.0 x 50 1.0 x 50 1.0 x 50 1.0 x 50 1.0 x 50 1.0 x 50 1.0 x 50 1.0 x 50 1.0 x 50 1.0 x 50 1.0 x 50 1.0 x 50 1.0 x 50 1.0 x 50 1.0 x 50 1.0 x 50 1.0 x 50 1.0 x 50 1.0 x 50 1.0 x 50 1.0 x 50 1.0 x 50 1.0 x 50

Product name	Size(mm) ID x Length
AAB-1T AAF-1T AAM-1T AAP-1T AAV-1T ABA-1T ABT-1T ACA-1T ACB-1T ADS-1T AED-1T AGA-1T AGE-1T AGT-1T AHR-1T AIA-1T ALC-1T ALS-1T ANA-1T AOV-1T APA-1T APB-1T APD-1T APE-1T APG-1T APH-1T APR-1T ARC-1T AST-1T AWG-1T	1.0 x 35 1.0 x 35 1.0 x 35 1.0 x 35 1.0 x 35 1.0 x 35 1.0 x 35 1.0 x 35 1.0 x 35 1.0 x 35 1.0 x 35 1.0 x 35 1.0 x 35 1.0 x 35 1.0 x 35 1.0 x 35 1.0 x 35 1.0 x 35 1.0 x 35 1.0 x 35 1.0 x 35 1.0 x 35 1.0 x 35 1.0 x 35 1.0 x 35 1.0 x 35 1.0 x 35 1.0 x 35 1.0 x 35 1.0 x 35

Product name	Size(mm) ID x Length
AAB-M8B AAF-M8B AAM-M8B AAP-M8B AAV-M8B ABA-M8B ABT-M8B ACA-M8B ACB-M8B ADS-M8B AED-M8B AGA-M8B AGE-M8B AGT-M8B AHR-M8B AIA-M8B ALC-M8B ALS-M8B ANA-M8B AOV-M8B APA-M8B APB-M8B APD-M8B APE-M8B APG-M8B APH-M8B APR-M8B ARC-M8B AST-M8B AWG-M8B	0.8 x 50 0.8 x 50 0.8 x 50 0.8 x 50 0.8 x 50 0.8 x 50 0.8 x 50 0.8 x 50 0.8 x 50 0.8 x 50 0.8 x 50 0.8 x 50 0.8 x 50 0.8 x 50 0.8 x 50 0.8 x 50 0.8 x 50 0.8 x 50 0.8 x 50 0.8 x 50 0.8 x 50 0.8 x 50 0.8 x 50 0.8 x 50 0.8 x 50 0.8 x 50 0.8 x 50 0.8 x 50 0.8 x 50 0.8 x 50

Product name	Size(mm) ID x Length
AAB-M8T AAF-M8T AAM-M8T AAP-M8T AAV-M8T ABA-M8T ABT-M8T ACA-M8T ACB-M8T ADS-M8T AED-M8T AGA-M8T AGE-M8T AGT-M8T AHR-M8T AIA-M8T ALC-M8T ALS-M8T ANA-M8T AOV-M8T APA-M8T APB-M8T APD-M8T APE-M8T APG-M8T APH-M8T APR-M8T ARC-M8T AST-M8T AWG-M8T	0.8 x 35 0.8 x 35 0.8 x 35 0.8 x 35 0.8 x 35 0.8 x 35 0.8 x 35 0.8 x 35 0.8 x 35 0.8 x 35 0.8 x 35 0.8 x 35 0.8 x 35 0.8 x 35 0.8 x 35 0.8 x 35 0.8 x 35 0.8 x 35 0.8 x 35 0.8 x 35 0.8 x 35 0.8 x 35 0.8 x 35 0.8 x 35 0.8 x 35 0.8 x 35 0.8 x 35 0.8 x 35 0.8 x 35 0.8 x 35

Product name	Size(mm) ID x Length
AAB-M5B AAF-M5B AAM-M5B AAP-M5B AAV-M5B ABA-M5B ABT-M5B ACA-M5B ACB-M5B ADS-M5B AED-M5B AGA-M5B AGE-M5B AGT-M5B AHR-M5B AIA-M5B ALC-M5B ALS-M5B ANA-M5B AOV-M5B APA-M5B APB-M5B APD-M5B APE-M5B APG-M5B APH-M5B APR-M5B ARC-M5B AST-M5B AWG-M5B	0.5 x 50 0.5 x 50 0.5 x 50 0.5 x 50 0.5 x 50 0.5 x 50 0.5 x 50 0.5 x 50 0.5 x 50 0.5 x 50 0.5 x 50 0.5 x 50 0.5 x 50 0.5 x 50 0.5 x 50 0.5 x 50 0.5 x 50 0.5 x 50 0.5 x 50 0.5 x 50 0.5 x 50 0.5 x 50 0.5 x 50 0.5 x 50 0.5 x 50 0.5 x 50 0.5 x 50 0.5 x 50 0.5 x 50 0.5 x 50

Product name	Size(mm) ID x Length
AAB-M5T AAF-M5T AAM-M5T AAP-M5T AAV-M5T ABA-M5T ABT-M5T ACA-M5T ACB-M5T ADS-M5T AED-M5T AGA-M5T AGE-M5T AGT-M5T AHR-M5T AIA-M5T ALC-M5T ALS-M5T ANA-M5T AOV-M5T APA-M5T APB-M5T APD-M5T APE-M5T APG-M5T APH-M5T APR-M5T ARC-M5T AST-M5T AWG-M5T	0.5 x 35 0.5 x 35 0.5 x 35 0.5 x 35 0.5 x 35 0.5 x 35 0.5 x 35 0.5 x 35 0.5 x 35 0.5 x 35 0.5 x 35 0.5 x 35 0.5 x 35 0.5 x 35 0.5 x 35 0.5 x 35 0.5 x 35 0.5 x 35 0.5 x 35 0.5 x 35 0.5 x 35 0.5 x 35 0.5 x 35 0.5 x 35 0.5 x 35 0.5 x 35 0.5 x 35 0.5 x 35 0.5 x 35 0.5 x 35

Product name	Size(mm) ID x Length
AAB-M3B AAF-M3B AAM-M3B AAP-M3B AAV-M3B ABA-M3B ABT-M3B ACA-M3B ACB-M3B ADS-M3B AED-M3B AGA-M3B AGE-M3B AGT-M3B AHR-M3B AIA-M3B ALC-M3B ALS-M3B ANA-M3B AOV-M3B APA-M3B APB-M3B APD-M3B APE-M3B APG-M3B APH-M3B APR-M3B ARC-M3B AST-M3B AWG-M3B	0.3 x 50 0.3 x 50 0.3 x 50 0.3 x 50 0.3 x 50 0.3 x 50 0.3 x 50 0.3 x 50 0.3 x 50 0.3 x 50 0.3 x 50 0.3 x 50 0.3 x 50 0.3 x 50 0.3 x 50 0.3 x 50 0.3 x 50 0.3 x 50 0.3 x 50 0.3 x 50 0.3 x 50 0.3 x 50 0.3 x 50 0.3 x 50 0.3 x 50 0.3 x 50 0.3 x 50 0.3 x 50 0.3 x 50 0.3 x 50

Product name	Size(mm) ID x Length
AAB-M3T AAF-M3T AAM-M3T AAP-M3T AAV-M3T ABA-M3T ABT-M3T ACA-M3T ACB-M3T ADS-M3T AED-M3T AGA-M3T AGE-M3T AGT-M3T AHR-M3T AIA-M3T ALC-M3T ALS-M3T ANA-M3T AOV-M3T APA-M3T APB-M3T APD-M3T APE-M3T APG-M3T APH-M3T APR-M3T ARC-M3T AST-M3T AWG-M3T	0.3 x 35 0.3 x 35 0.3 x 35 0.3 x 35 0.3 x 35 0.3 x 35 0.3 x 35 0.3 x 35 0.3 x 35 0.3 x 35 0.3 x 35 0.3 x 35 0.3 x 35 0.3 x 35 0.3 x 35 0.3 x 35 0.3 x 35 0.3 x 35 0.3 x 35 0.3 x 35 0.3 x 35 0.3 x 35 0.3 x 35 0.3 x 35 0.3 x 35 0.3 x 35 0.3 x 35 0.3 x 35 0.3 x 35 0.3 x 35