Shodex: Packed Columns for HPLC Analysis

Chiral Separation Columns (ORpak CD)

Besides the positive and negative properties of optical rotation, optical isomers have the same physical and chemical characteristics. Optical separation columns are indispensable for direct analysis without derivatization. Shodex ORpak series incorporate packing material bonded by ligands that can identify many optical isomers.
ORpak series has the following ligands: cyclodextrin derivative (CD).
Because CD is used as the ligand, these columns are suitable for the analysis of optical isomers that make up an inclusion complex with CD. alpha-CD is used as the ligand in CDA, beta-CD is used as the ligand in CDB and CDBS and gamma-CD is used as the ligand in CDC. As a result, different columns can be used based on the size of the hydrophobic area of the optical isomer that is included. Packing materials in CDB and CDBS columns are different. Since CDBS is packed with silica gel, the peaks are sharp and many optical isomers can be analysed. Since CDB is packed with polymer-based gel, a wide range of separation conditions is possible, including alkaline separation. Since beta-CD identifies the most optical isomers within the CD's, CDB and CDBS are suitable for preliminary studies of optical isomer analysis. The CD series are suitable for the analysis of, not only optical isomers, but also structural isomers that have similar structure.

(Standard columns)

Product name	Liand	Particle size (micron)	Size(mm) ID x Length
ORpak CDA-453 HQ ORpak CDB-453 HQ ORpak CDC-453 HQ ORpak CDBS-453 ORpak CD-G	alpha-Cyclodextrin derivertive beta-Cyclodextrin derivertive gamma-Cyclodextrin derivertive beta-Cyclodextrin derivertive Guard column	6 6 6 3 -	4.6 x 150 4.6 x 150 4.6 x 150 4.6 x 150 4.6 x 10

Product name	Max. usable pressure (MPa)	Max. usable flow rate (mL/min)	Usable temp. range (deg-C)	Usable pH range	Usable salt conc.	In-column solvent
CDA-453 HQ CDB-453 HQ CDC-453 HQ CDBS-453 CD-G	4 4 4 20 -	1.0 1.0 1.0 1.2 -	4 to 60 4 to 60 4 to 60 4 to 60 4 to 60	3 to 10 3 to 10 3 to 10 2 to 7 3 to 10	< 3M < 3M < 3M < 1M < 3M	H2O H2O H2O CH3CN/H2O=95/5 H2O