Shodex: Packed Columns for HPLC Analysis

Ligand Exchange Columns for Saccharides: SUGAR S, USPpak

Saccharides are separated by combining different separation modes, such as ligand exchange, size exclusion, ion exclusion and partition/adsorption. Suitable for separation of sugar and sugar alcohol.

(Standard Columns)

Product name	Counter ion	Separation mode	Exclusion limit (Pullulan)	Plate number (TPN/column)	Size(mm) ID x Length
SUGAR SH1011 SUGAR SH1821 SUGAR SH-G	H+ H+ H+	SEC+IEX SEC+IEX Guard column	1,000 10,000 -	> 17,000 > 17,000 -	8.0 x 300 8.0 x 300 6.0 x 50
SUGAR SC1011 SUGAR SC1821 SUGAR SC-LG	Ca2+ Ca2+ Ca2+	SEC+LEX SEC+LEX Guard column	1,000 10,000 -	> 13,000 > 13,000 -	8.0 x 300 8.0 x 300 6.0 x 50
SUGAR SP0810 SUGAR SP-G	Pb2+ Pb2+	SEC+LEX Guard column	1,000 -	> 11,000 -	8.0 x 300 6.0 x 50
SUGAR SC1211 SUGAR SC-G	Ca2+ Ca2+	N&R+LEX Guard column	- -	> 5,500 -	6.0 x 250 4.6 x 10
SUGAR SZ5532 SUGAR SZ-G	Zn2+ Zn2+	N&R+LEX Guard column	- -	> 5,500 -	6.0 x 150 4.6 x 10
USPpak MN-431	Ca2+	SEC+LEX	-	> 4,000	4.0 x 250

Product name	Particle size (micron)	Max. usable pressure (MPa)	Recommended flow rate (mL/min)	Max. flow rate (mL/min)	Usable temp. (deg-C)	In-column solvent
SH1011 SH1821 SH-G	6 6 10	5 5 -	0.5 to 1.0 0.5 to 1.0 0.5 to 1.0	1.5 1.5 -	< 95 < 95 < 95	H2O H2O H2O
SC1011 SC1821 SC-LG	6 6 10	4 4 -	0.5 to 1.0 0.5 to 1.0 0.5 to 1.0	1.5 1.5 -	< 95 < 95 < 95	H2O H2O H2O
SP0810 SP-G	7 10	3 -	0.5 to 1.0 -	1.0 -	< 95 < 95	H2O H2O
SC1211 SC-G	6 10	4 -	0.5 to 1.0 -	1.0 -	< 95 < 95	25%CH3CN/H2O 25%CH3CN/H2O
SZ5532 SZ-G	6 6	4 -	0.5 to 1.0 -	2.0 -	< 80 < 80	70%CH3CN/H2O 70%CH3CN/H2O
MN-431	-	3.5	-	0.6(1)	30 to 85	H2O

*0.6mL/min: when column temperature range is 51 to 85 deg-C.
0.5mL/min: when column temperature range is 31 to 50 deg-C.
0.3mL/min: when column temperature range is below 30 deg-C.
Separation mode:
SEC=Size exclusion, IEX=Ion exchange, LEX=Ligand exchange, N&R=Normal & reversed phase
Exclusion limit:
Measuered using pullulan standards.
Combination of one type of column with another:
When separation cannot be satisfactorily performed, use in series of an additional column of the same or a different type will improve separation. Such combination, however, is possible only among SP0810 and columns of the SC and KS series, and they must always be arranged in the following order.
SUGAR KS column + SUGAR SC column + SUGAR SP0810 When a guard column is used for any combination of columns, it must be one designed for use with the first column in the combination.
Pretreatment of sample:
The sample is usually dissolved in the eluent. Pass the dissolved sample through a 0.45micron membrane filter and inject it into the column. Use of Shodex DT disposable filter unit is recommended. Any proteins contained in the sample must be removed before filtering.
Samples containing heavy metal ions, surfactants (emulsifiers) or oily substances that adsorb on the packing should be passed through a mass of H-type cation exchange resin before filtering.
Organic acids can be removed by passing the sample through a mass of anion-exchange resin. In the case of columns of the SH group, however, samples containing organic acids require no pretreatment. Alkaline samples must be neutralized before injection; otherwise, column performance will deteriorate.
Eluent conditions:
The water used as the eluent must be deionized water that has been passed through a 0.45micron membrane filter.
Before use, it must also be degassed without fail to prevent bubbling in the column and at its outlet, because all columns of this series are heated being used. Use of degassing devices of the Shodex DEGAS is recommended.

SH type:
Although deionized water can be used alone as the eluent, adsorption of alkalis on the packing material will change the chromatogram. Addition of sulfuric or phosphoric acid to the mobile phase is, therefore1 recommended to prevent that change.
SC type and SP0810:
Basically, deionized water is used as the eluent. A polar organic solvent, if added to the eluent, will improve separation of sugar alcohols and reduce hydrophobic adsorption. The concentration of the polar organic solvent must, however, be 20% maximum.
SC type:
Addition of a salt to the eluent will improve separation of ionic substaces. The salt must be either Ca(NO₃)₂ or CaSO₄, and its pH, in the 3 to 7 range. Also, in the case of analysis of samples containing large quantities of heavy metals, addition of about 10 to 50 ppm of Ca-EDTA will prevent deterioration of column performance.
SC1211 and SZ5532:
The eluent used with SC1211 and SZ5532 is basically a mixture of water and an organic solvent, generally acetonitrile, and it is used in partition chromatography.