GFC & Ligand Exchange Columns for Saccharides: SUGAR KS-800

Saccharides are separated by combining size separation and ligand exchange. Since we provide columns that differ in molecular weight of exclusion limit, column selection can be made based on molecular weight of monosaccharide, disaccharide, oligosaccharide and polysaccharide.

(Standard Columns)
Product name
Counter ion
Separation
mode
Exclusion limit
(Pullulan)
Plate number
(TPN/column)
Size(mm)
ID x Length
SUGAR KS-801
SUGAR KS-802
SUGAR KS-803
SUGAR KS-804
SUGAR KS-805
SUGAR KS-806
SUGAR KS-G
Na+
Na+
Na+
Na+
Na+
Na+
Na+
SEC+LEX
SEC+LEX
SEC
SEC
SEC
SEC
Guard column
1,000
10,000
50,000
400,000
5,000,000
(50,000,000)
-
> 17,000
> 17,000
> 17,000
> 17,000
> 9,000
> 9,000
-
8.0 x 300
8.0 x 300
8.0 x 300
8.0 x 300
8.0 x 300
8.0 x 300
6.0 x 50
new SUGAR KS-807
new SUGAR KS-807G
Na+
Na+
SEC
Guard column
(200,000,000)
-
> 4,000
-
8.0 x 300
8.0 x 50
Product
name
Particle size
(micron)
Max. usable
pressure
(MPa)
Max. usable
flow rate
(mL/min)
Usable
temp.
(deg-C)
In-column
solvent
KS-801
KS-802
KS-803
KS-804
KS-805
KS-806
KS-G
6
6
6
7
17
17
10
5
5
5
5
5
5
-
1.5
1.5
1.5
1.5
1.5
1.5
-
< 85
< 85
< 85
< 85
< 85
< 85
< 85
H2O
H2O
H2O
H2O
H2O
H2O
H2O
KS-807
KS-807G
30
30
0.5
-
1.5
-

< 85
< 85

H2O
H2O

Separation mode:
SEC: Size exclusion
LEX: Ligand exchange
Exclusion limit:
Measured with pullulan.
Eluent conditions:
1) Use distilled water as the eluent.
2) Remove extraneous substances and insolubles from the eluent by passing through 0.45 micron filter into a bottle and throughly degas it by, for instance, heating to about 60 deg-C in a water bath and shaking in an ultrasonic washing vessel with the ambient air pressure being simulataneously reduced by an aspirator. Use of a solvent degassing device of ERC degasser will facilitate the degassing work. 3) Adsorbed sometimes by the packing material, molecules of highly hydrophobic samples are to too slow to flow out of the column. In such a case, add polar organic solvent to the eluent to reduce the adsorptivity. For KS-801 to KS-803, polar organic solvent is added in an amount of 20% maximum to the eluent and for KS-804 to KS-807, polar organic solvent is added in an amount of 50% maximum to the eluent.

(Preparative Columns)(Remark)
Product name Plate number
(TPN/column)
Particle size
(micron)
Size(mm)
ID x Length
Standard
column
SUGAR KS-2001
SUGAR KS-2002
SUGAR KS-2003
SUGAR KS-2004
SUGAR KS-2005
SUGAR KS-2006
SUGAR KS-LG
> 7,000
> 7,000
> 8,000
> 6,000
> 6,000
> 6,000
-
20
20
20
18
25
25
20
20.0 x 300
20.0 x 300
20.0 x 300
20.0 x 300
20.0 x 300
20.0 x 300
8.0 x 50
KS-801
KS-802
KS-803
KS-804
KS-805
KS-806
Guard column

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