Shodex: Packed Columns for HPLC Analysis

Silica-based GFC Columns: PROTEIN KW-800

GFC columns are suitable for analysis of high molecular weight biological fluids such protein and for molecular weight determination. At Shodex, we provide GFC columns: KW series , SB-800 HQ series, GS-HQ series and GF-HQ series . Selection can be made to meet your analytical needs. Compared with polymer-based columns, KW series are suitable for separating molecules, such as protein, with molecular weight from a few thousand to a few million. In addition to standard columns with ID 8.0mm, we provide micro columns with ID 300micron-800micron, semimicro columns with ID 1.0mm-4.6mm and preparative columns with ID 10mm and above.

(Standard Columns)

Product name	Exclusion limit		Plate number (TPN/column)	Particle size (micron)	Size(mm) ID x Length
	(Pullulan)	(Proteins)
PROTEIN KW-802.5	60,000	150,000	> 21,000	5	8.0 x 300
PROTEIN KW-803	170,000	700,000	> 21,000	5	8.0 x 300
PROTEIN KW-804	500,000	1,000,000	> 16,000	7	8.0 x 300
PROTEIN KW-604S	For column switching		> 800	7	6.0 x 50
PROTEIN KW-G	Guard column		-	7	6.0 x 50

For all products
Recommended flow rate: < 1.0mL/min
Max. usable flow rate: 1.5mL/min
Max. usable pressure: 5MPa
Max. usable temperature: 45deg-C
Usable pH range: 3.0 to 7.5
Usable salt conc. in eluent: 0.1 to 0.3M
Usable organic solvent concentration: Up to 100% for methanol and acetonitrile.

(Micro column and Semimicro columns)(Remark)

Product name	Standard column	Product name	Standard column	Product name	Standard column	Size(mm) ID x Length
KW802.5-4E KW802.5-4D KW802.5-4B KW802.5-2E KW802.5-2D KW802.5-2B KW802.5-1E KW802.5-1D KW802.5-1B	KW-802.5	KW803-4E KW803-4D KW803-4B KW803-2E KW803-2D KW803-2B KW803-1E KW803-1D KW803-1B	KW-803	KW804-4E KW804-4D KW804-4B KW804-2E KW804-2D KW804-2B KW804-1E KW804-1D KW804-1B	KW-804	4.6 x 250 4.6 x 150 4.6 x 50 2.0 x 250 2.0 x 150 2.0 x 50 1.0 x 250 1.0 x 150 1.0 x 50
KW802.5-M8E KW802.5-M8D KW802.5-M8B KW802.5-M5E KW802.5-M5D KW802.5-M5B KW802.5-M3E KW802.5-M3D KW802.5-M3B	KW-802.5	KW803-M8E KW803-M8D KW803-M8B KW803-M5E KW803-M5D KW803-M5B KW803-M3E KW803-M3D KW803-M3B	KW-803	KW804-M8E KW804-M8D KW804-M8B KW804-M5E KW804-M5D KW804-M5B KW804-M3E KW804-M3D KW804-M3B	KW-804	0.8 x 250 0.8 x 150 0.8 x 50 0.5 x 250 0.5 x 150 0.5 x 50 0.3 x 250 0.3 x 150 0.3 x 50

(Preparative Columns)(Remark)

Product name	Plate number (TPN/column)	Particle ize (micron)	Size(mm) ID x Length	Standard column
PROTEIN KW-2002.5	> 17,000	5	20.0 x 300	KW-802.5
PROTEIN KW-2003	> 17,000	5	20.0 x 300	KW-803
PROTEIN KW-2004	> 14,000	7	20.0 x 300	KW-804
PROTEIN KW-LG	Guard column	7	8.0 x 50	Guard column