Shodex: Packed Columns for HPLC Analysis

Standards for GFC

The analysis of molecular-weight distribution of polymers using GPC and GFC columns requires the calibration curve development to obtain information on molecular weights of samples of interest. Shodex STANDARDs are standard samples for calibration curve development. Polystyrene and Poly(methylmethacrylate) for GPC with organic solvents, including HFIP, and pullulan for GFC are available, from which you can select the most suitable one for the purpose.

Note: In the same type of Shodex STANDARDs, molecular weights (Mw, Mw/Mn, and Mp) may be varied, depending on production lots.

STANDARD P-82
Standard sample of fractionated, purified not-branched pullulan. This is easily soluble in water. Once dissolved, it does not recrystallize.

STANDARD P-82
Std.No.	Mw	Mw/Mn
P-800 P-400 P-200 P-100 P-50 P-20 P-10 P-5	788,000 404,000 212,000 112,000 47,300 22,800 11,800 5,900	1.23 1.13 1.13 1.12 1.06 1.07 1.10 1.07

(Structual formula of P series)


Mw was determined by ultracentrifugation sedimentation equilibrium method and Mw/Mn was determined by GFC. Courtesy of Professor Kazuo Kawahara, the Faculty of Pharmacy, Nagasaki University.

(References)
(1) K.Kawahara and S.Nakamura, Carbohydrate Polymers, 4 (1984) 335.
(2) T.Kato, T. Tokuda, A.Takahashi, Biopolymers, 21 (1982) 1623.