Shodex: Packed Columns for HPLC Analysis

Comparison of Sample Retention Using ODP, C8P and C4P Columns

The difference of retention of three columns for reversed phase chromatography, Asahipak ODP, C8P and C4P were compared.


Sample :
1. beta-Endorphin
2. Big gastrin
3. Insulin

Columns      : Shodex Asahipak ODP-50, C8P-50, C4P-50 (4.6mmID*150mm each)
Eluent       : 0.05% TFA aq./CH3CN=72/28
Flow rate    : 0.6mL/min
Detector     : UV(220nm)
Column temp. : 30deg-C