Shodex: Packed Columns for HPLC Analysis

Recovery of Proteins and Enzyme Activity (DEAE-825)

The tables show good recovery of IEC DEAE-825 (a column for ion exchange chromatography) for proteins and enzyme activity.

Recovery of proteins
Protein	Recovery (%)
Trypsin inhibitor	96
beta-Lactoglobulin	100
Ovalbumin	101
Myoglobin	100
gamma-Globulin	99
BSA	97
Thyroglobulin	90

Recovery of enzyme activity
Enzyme	Recovery (%)
Kallikrein	92
Lipoxidase	96
Catalase	94

Sample load : 0.5mg each

Column : Shodex IEC DEAE-825 (8.0mmID*75mm)
Eluent : (A); 20mM Tris-HCl buffer(pH8.0)
         (B); (A) + 0.5M NaCl
         Linear gradient: 0min to 1min, (A) to (B)