Shodex: Packed Columns for HPLC Analysis

Standard Proteins (8) (ES-502C 7C)

Four kinds of protien standard were analyzed using Asahipak ES-502C 7C ( a column for weak cation exchange chromatography ).


Sample :
1. Myoglobin
2. Ribonuclease A
3. Chymotrypsinogen A
4. Lysozyme

Column    : Shodex Asahipak ES-502C 7C (7.6mmID*100mm)
Eluent    : (A); 50mM Sodium phosphate buffer(pH7.0)
            (B); (A) + 0.5M NaCl
            Linear gradient: 0min to 20min, (A) to (B)
Flow rate : 1.0mL/min
Detector  : Shodex UV(280nm)