Shodex: Packed Columns for HPLC Analysis

Human Serum (4) (DEAE-825)

Human serum was analyzed using IEC DEAE-825 ( a column for waek anion exchange chromatography ).


Sample : Human serum

Column       : Shodex IEC DEAE-825 (8.0mmID*75mm)
Eluent       : (A); 0mM Tris-HCl buffer(pH8.0)
               (B); (A) + 0.5M NaCl 
               Linear gradient: 0min to 60min, (A) to (B)
Flow rate    : 1.0mL/min
Detector     : Shodex UV(280nm)
Column temp. : Room temp.