Shodex: Packed Columns for HPLC Analysis

Proteins in Human Serum (2) (DEAE-825)

Proteins in human serum were analyzed using IEC DEAE-825 ( a column for weak anion exchange chromatography ).


Sample : Human Serum
1. Albumin
2. alpha1 Acid glycoprotein
3. Transferrin
4. IgA
5. IgM
6. IgG

Column       : Shodex IEC DEAE-825 (8.0mmID*75mm)
Eluent       : (A); 20mM Tris-CH3COOH buffer(pH8.0)
               (B); (A) + 0.5M CH3COONa
               Linear gradient: 0min to 60min, (A) to (B)
Flow rate    : 1.0mL/min
Detector     : Shodex UV(280nm)
Column temp. : Room temp.