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Collagen and BSA

Collagen and BSA were analyzed using Asahipak ES-502N 7C (a column for weak anion exchange chromatography).


Sample :
1. Collagen
Pepsin-digested product of collagen
soluble in acid extracted from pig skin
2. BSA

Column       : Shodex Asahipak ES-502N 7C (7.6mmID*100mm)
Eluent       : 20mM Monoethanolamine buffer(pH9.5) + 500mM NaCl 
Flow rate    : 1.0mL/min
Detector     : Shodex UV(280nm)
Column temp. : 30deg-C

Courtesy of Dr. Shirai, Tokyo University of Agriculture and Technology