Shodex: Packed Columns for HPLC Analysis

Myosin Subfragments

Myosin subfragments were analyzed using Asahipak ES-502C 7C ( a column for weak cation exchange chromatography ).


Sample : Myosin subfragment

Column       : Shodex Asahipak ES-502C 7C (7.6mmID*100mm)
Eluent       : (A); 5mM Sodium phosphate buffer(pH7.0)
               (B); (A) + 0.1M NaCl
               Linear gradient: 0min to 25min, (A) to (B)
Flow rate    : 1.0mL/min
Detector     : Shodex UV(220nm)
Column temp. : 10deg-C

Courtesy of Dr. Muno, Juntendo University