Shodex: Packed Columns for HPLC Analysis

Purified Kallikrein from Pig Pancreas

Purified Kallikrein from Pig Pancreas was analyzed using IEC QA-825 ( a column for strong anion exchange chromatography ). Enzyme activity was found at the large peak which has two peak tops after 30 minutes. The recovery of enzyme activity was 95%.


Sample : 0.5% Kallikrein, 200micro-L

Column       : Shodex IEC QA-825 (8.0mmID*75mm)
Eluent       : (A); 20mM Tris-HCl buffer(pH7.5)
               (B); (A) + 0.5M NaCl
               Linear gradient: 0min to 60min, (A) to (B)
Flow rate    : 1.0mL/min
Detector     : Shodex UV (280nm)
Column temp. : 25deg-C