Shodex: Packed Columns for HPLC Analysis

Lipoxidase (2) (SP-420N)

Lipoxidase was analyzed using IEC SP-420N (a column for strong cation exchange chromatography packed with non-porous gel).


Sample : Lipoxidase

Column       : Shodex IEC SP-420N (4.6mmID*35mm)
Eluent       : (A); 20mM MES* buffer(pH6.0)
               (B); (A) + 0.5M Na2SO4
               Linear gradient: 0min to 15min, (A) to (B)
Flow rate    : 1.5mL/min
Detector     : Shodex UV(280nm)
Column temp. : Room temp.

MES: 2-(N-Morpholino) ethanesulfonic acid