Shodex: Packed Columns for HPLC Analysis

Carcinogenic Tryptophan Pyrolysis Products

Carcinogenic tryptophan pyrolysis products were analyzed using Asahipak ES-502C 7C (a column for weak cation exchange chromatography).


Sample :
Trp-P-1
Trp-P-2

Column       : Shodex Asahipak ES-502C 7C (7.6mmID*100mm)
Eluent       : 20mM H3PO4(pH2.0)/CH3CN=90/10
Flow rate    : 1.0mL/min
Detector     : Fluorescence detector(Ex. 266nm, Em. 397nm)
Column temp. : 40deg-C

S Manabe and O. Wada, Exp. Eye Res., 48 (1989) 351.