Shodex: Packed Columns for HPLC Analysis

Histidine and 3-Methylhistidine

Histidine and 3-methylhistidine were analyzed using Asahipak ODP-50 6D. Analysis by the reversed phase mode which makes use of difference in hydrophobicity is commonly used for analyzing amino acid derivatives.


Sample :
1. Histidine
2. 3-Methylhistidine

Column       : Shodex Asahipak ODP-50 6D (6.0mmID*150mm)
Eluent       : 10mM Sodium phosphate buffer(pH7.0)
Flow rate    : 0.5mL/min
Detector     : Shodex UV(210nm)
Column temp. : 30deg-C