Shodex: Packed Columns for HPLC Analysis

Nucleotides (10) (WA-624 pH5.5)

Nucleotides were analyzed using AXpak WA-624 (a column for weak anion exchange chromatography). pH of the eluent is heightened to 5.5.
Please compare with the cases of pH4.3 and pH5.0


Sample :
1. 5'CDP
2. 5'UDP
3. 5'ADP
4. 5'GDP

Column       : Shodex AXpak WA-624 (6.0mmID*150mm)
Eluent       : 0.7M CH3COONH4 buffer(pH5.5)
Flow rate    : 1.0mL/min
Detector     : Shodex UV(260nm)
Column temp. : 60deg-C