Shodex: Packed Columns for HPLC Analysis

DNA Fragments Digested by Hae III (DEAE-420N)

DNA fragments digested by Hae III were analyzed using IEC DEAE-420N (a column for weak anion exchange chromatography packed with non-porous gel).

Sample : phi-X-174-RF DNA fragment

No.	base pairs	MW (x 10⁵daltons)
1.	1353	8.79
2.	1078	7.01
3.	872	5.67
4.	603	3.92
5.	310	2.02
6B.	281	1.83
6A.	271	1.76
7.	234	1.52
8.	194	1.26
9.	118	0.77
10.	72	0.47

Column       : Shodex IEC DEAE-420N (4.6mmID*35mm)
Eluent       : (A); 25mM Phosphate buffer(pH5.2) 
               (B); (A) + 1M NaCl
               Linear gradient: 0min to 20min, 75% (B) to 100% (B) 
Flow rate    : 1.0mL/min
Detector     : UV(260nm)
Column temp. : 30deg-C