Shodex: Packed Columns for HPLC Analysis
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Separation of Anomer
It has been reported that the anomer separation does not take place at high temperature.
Therefore, when using SUGAR series (
SP0810,
SC1011,
SC1211 or
KS-800), set up the column
temperature at 70-80deg-C.
Please refer to
Prevention of Anomer Separation.

Sample :
G
lucose
Galactose
Mannose
XyloseArabinose
Column : Shodex SUGAR SC1211 (6.0mmID*250mm)
Eluent : H2O
Flow rate : 0.7mL/min
Detector : Shodex RI
Column temp. : (A); 70deg-C, (B); 25deg-C