Monosaccharides and Oligosaccharides Analysis = Separation of Anomer

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Separation of Anomer

It has been reported that the anomer separation does not take place at high temperature. Therefore, when using SUGAR series (SP0810, SC1011, SC1211 or KS-800), set up the column temperature at 70-80deg-C.
Please refer to Prevention of Anomer Separation.

Sample :
G lucose
Galactose
Mannose
Xylose
Arabinose

Column       : Shodex SUGAR SC1211 (6.0mmID*250mm)
Eluent       : H₂O
Flow rate    : 0.7mL/min
Detector     : Shodex RI
Column temp. : (A); 70deg-C, (B); 25deg-C

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