Shodex: Packed Columns for HPLC Analysis

Separation of Anomer

It has been reported that the anomer separation does not take place at high temperature. Therefore, when using SUGAR series (SP0810, SC1011, SC1211 or KS-800), set up the column temperature at 70-80deg-C.
Please refer to Prevention of Anomer Separation.


Sample :
G lucose
Galactose
Mannose
Xylose
Arabinose

Column       : Shodex SUGAR SC1211 (6.0mmID*250mm)
Eluent       : H2O
Flow rate    : 0.7mL/min
Detector     : Shodex RI
Column temp. : (A); 70deg-C, (B); 25deg-C