Durability against Alkaline Solvents
Since the packing material of Asahipak
NH2P-50 column is polymer, it is durable against alkaline solvents. To the contrary, silica-based amino columns are not durable against alkaline solvents.
Testing procedure
(1)Equilibrium(1hr) Conditions before testing
Eluent : 100mM Ammonium acetate(pH9.3)
Flow rate : 0.1mL/min
Column temp. : 25deg-C
|
(2)Testing(160hrs)
Eluent : 5mM NaOH(pH11.4)/CH3CN=25/75
Flow rate : 0.1mL/min
Column temp. : 30deg-C
|
(3)Replacement(0.5hr)
Eluent : H2O
Flow rate : 0.5mL/min
Column temp. : 25deg-C
|
(4)Equilibrium(1hr)
Eluent : 100mM Ammonium acetate(pH9.3)
Flow rate : 0.5mL/min
Column temp. : 25deg-C |
Comparison between before and after testing
| Sample | Before testing | After testing |
|---|
Elution volume
(mL) | Plate number |
Elution volume
(mL) | Plate number |
| 1.Fructose | 7.52 | 7100 |
7.62 | 8100 |
| 2.Glucose | 9.63 | 6100 |
9.68 | 7700 |
| 3.Sucrose | 13.41 | 8400 |
13.40 | 9700 |
| 4.Maltose | 16.16 | 7200 |
16.08 | 7400 |
Column : Shodex Asahipak NH2P-50 4E (4.6mmID*250mm)
Eluent : CH3CN/H2O=75/25
Flow rate : 1.0mL/min
Detector : Shodex RI
Column temp. : 30deg-C
