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Monosaccharides (3)

Monosaccharides were analyzed using Asahipak NH2P-50 4E (a column for saccharides analysis).


Sample : 10micro-L
1. 20mg/mL Mannose
2. 20mg/mL Galactose
3. 1mg/mL Glucose

Column       : Shodex Asahipak NH2P-50 4E (4.6mmID*250mm)
Eluent       : 10mM *Tetrapropylammonium buffer(pH10.0)/CH3CN=20/80
Flow rate    : 0.6mL/min
Detector     : Shodex RI
Column temp. : 30deg-C

^*How to make Tetrapropylammonium buffer
Please add acetic acid to 10mM Tetrapropylammonium hydroxide and adjust pH10.