High-sensitive, High-selective Analysis of Saccharides = Derivertization of Neutral Sugars by Pyridylamine

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Derivertization of Neutral Sugars by Pyridylamine

This is an example of the fluorescence detection of pyridylamino-saccharides. Asahipak NH2P-50 4E (a column for saccharides analysis) is used for the separation.

Sample :
1. 2-Aminopyridine
2. PA-Fucose
3. PA-Xylose
4. PA-Mannose
5. PA-Galactose
6. PA-Glucose

Column       : Shodex Asahipak NH2P-50 4E (4.6mmID*250mm)
Eluent       : [50mM Tris-HCl buffer + 25mM Boric acid(pH8.5)]/CH₃CN=20/100
Flow rate    : 0.5mL/min
Detector     : Fluorescence Detector(Ex: 320nm , Em: 400nm)
Column temp. : 50deg-C

Courtesy of Dr. Imanari, Chiba University

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