Shodex: Packed Columns for HPLC Analysis
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Local Anesthetics (1)
When using an alkaline eluent with a higher pH than the pKa sample value, basic
drug components which have tertiary amino groups can be retained by the column
because the amino groups are not dissociated and, in consequence, the hydrophilicity
of the drug components decrease. Such analysis conditions can be realized for
Asahipak
ODP-50 columns because they can be used at pH11.
Sample :
1.
Benzocaine,
2.
Lidocaine,
3.
Tetracaine hydrochloride
Column : Shodex Asahipak ODP-50 4D (4.6mmID*150mm)
Eluent : 25mM Phosphate buffer/CH3CN=60/40
Flow rate : 0.6mL/min
Detector : UV(254nm)
Column temp. : 30deg-C
