Cinchona Alkaloids
For the analysis of cinchona alkaloids, cinchonine and quinine, which are
basic drug components having tertiary amino groups, good separation can be obtained
with alkaline eluent at pH9.0 using an Asahipak
ODP-50 6D column. The separation at pH7.4 when using ODP or ODS columns is not
as good as that obtained at pH9.0 when using an ODP column.
Sample :
1.
Cinchonine,
2.
Quinine
Column : Shodex Asahipak ODP-50 6D (6.0mmID*150mm), ODS (other manufacturer)
Eluent : 10mM Phosphate buffer/CH3CN/CH3OH=40/20/40
Flow rate : 1.0mL/min
Detector : Shodex UV(250nm)
Column temp. : 30deg-C
