Shodex: Packed Columns for HPLC Analysis

Barbiturates

Barbiturates, which are widely used as hypnotics, have a maximum UV absorbance at 240nm when an alkaline eluent used. This characteristics make it possible to obtain a highly sensitive analysis for barbiturates using Asahipak ODP-50 4D (a column for reversed phase chromatography).


Sample :
1. Barbital
2. Phenobarbital
3. Hexobarbital
4. Pentobarbital
5. Secobarbital

Column       : Shodex Asahipak ODP-50 4D (4.6mmID*150mm)
Eluent       : 50mM Phosphate buffer(pH11.0)/CH3OH=75/25
Flow rate    : 0.5mL/min
Detector     : Shodex UV(254nm)
Column temp. : 30deg-C