Analysis of Metabolites = Polyamine and Acetylated Derivatives

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Polyamine and Acetylated Derivatives

Polyamine and acetylated derivatives were analyzed using Asahipak ODP-50 4E (a column for reversed phase chromatography) with alkaline eluent.

Sample :
1. Putrescine
2. Acetylputrescine
3. Cadaverine
4. Spermidine
5. Acetylcadaverine
6. N¹-Acetylspermidine
7. N⁸-Acetylspermidine
8. Spermine
9. Acetylspermine

Column       : Shodex Asahipak ODP-50 4E (4.6mmID*250mm)
Eluent       : 200mM Sodium phosphate (pH7.7) + 2.3mM Sodium 1-octanesulfonate 
Flow rate    : 0.5mL/min
Detector     : Fluoresence(Ex: 345nm, Em: 455nm) (OPA post-column reaction)
Column temp. : Room temp.

Courtesy of Dr. Kamei, The Tokyo Metropolitan Institute od Medical Science

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