Shodex: Packed Columns for HPLC Analysis

Fat-Soluble Vitamins (1) (ODP-50 4E)

Asahipak ODP-50 4E was used for the simultaneous analysis of fat-soluble vitamins. Good calibration curves were obtained for them.


Sample : 20micro-L
1. Vitamin K₃
2. Vitamin A
3. Vitamin A acetate
4. Vitamin D₂
5. Vitamin D₃
6. Vitamin E acetate
7. Vitamin E
8. Vitamin K₁

Column       : Shodex Asahipak ODP-50 4E (4.6mmID*250mm)
Eluent       : CH3CN/CH3OH=50/50
Flow rate    : 0.6mL/min
Detector     : Shodex UV(280nm)
Column temp. : 30deg-C