Catecholamines in Urine (ES-502C 7C)
Catecholamines in urine were analyzed using Asahipak
ES-502C 7C (a column for weak cation exchange chromatography).
Sample :
Catecholamines in
Urine
E.
Adrenaline
N.
Norepinephrine
D.
Dopamine
Column : Shodex Asahipak ES-502C 7C (7.6mmID*100mm)
Eluent : 0.05M Succinic acid + 0.15M sodium succinate + 0.5M EDTA(pH6.7)
Reaction reagent : (A); 0.1M glycylglycine containing 0.1M tartaric acid + 0.1M boric acid
+ 2mM zinc sulphate(pH6.5)
(B); 0.25M potassium borate buffer(pH8.4) containing 0.01%(w/v) hexacyanoferrate(III)
Flow rate : (Eluent) 1.0mL/min, (Reaction reagent) 0.35mL/min each
Detector : Fruorescence detector(Ex 350nm, Em 500nm)
Column temp. : 30deg-C
Reaction temp. : 90deg-C
T. Seki et al., The Chemical Sciety of Japan,
(7) (1986) 1040
