Shodex: Packed Columns for HPLC Analysis

Catecholamine and Salsolinol

Catecholamines and salsolinol were analyzed using Asahipak ES-502C 7C (a column for weak cation exchange chromatography) and an electrochemical detector (ECD).


Sample : Catecholamines
E. Epinephrine
NE. Norepinephrine
DA. Dopamine
SA. Salsolinol

Column       : Shodex Asahipak ES-502C 7C (7.6mmID*100mm)
Eluent       : 25mM Boric acid buffer + 75mM Succinic acid + 20mM EDTA(pH8.0)
Flow rate    : 1.0mL/min
Detector     : ECD(700mV, VS, Ag/AgCl)
Column temp. : 40deg-C

Courtesy of Dr. Chiba, Nihon University