Shodex: Packed Columns for HPLC Analysis

Catecholamines and Metabolites (1) (ODP-50 6D)

Catecholamines and metabolites were analyzed using Asahipak ODP-50 6D.


Sample : Catecholamines
NE: Norepinephrine
E: Epinephrine
DOPA: 3,4-Dihydroxyphenylalanine
DA: Dopamine
5HT: 5-Hydroxytryptamine
DOMA: 3,4-Dihydroxymandelic acid
MHPG: 3-Methoxy-4-hydroxyphenylglycol
VMA: Vanillylmandelic acid
DOPAC: 3,4-Dihydroxyphenylacetic acid
HVA: Homovanilic acid
5HIAA: 5-Hydroxyindole-3-acetic acid

Column       : Shodex Asahipak ODP-50 6D (6.0mmID*150mm)
Eluent       : (A); 0.1M H3PO4(pH2.0)
               (B); (A)/CH3CN=70/30
               Linear gradient is started at 10min with 2.5%/min gradient of (B).
Flow rate    : 0.8mL/min
Detector     : UV(280nm)