Shodex: Packed Columns for HPLC Analysis

LTs Produced by Polymorphonuclear Leukocyte of Rat

LTs produced by polymorphonuclear leukocyte of rat were analyzed using Asahipak ODP-50 6D ( a column for reversed phase chromatography ).


Sample : Leukotrienes

Column       : Shodex Asahipak ODP-50 6D (6.0mmID*150mm)
Eluent       : 0.2M CH3COONa buffer(pH4.5 adjusted with CH3COOH)/CH3CN/CH3OH=50/25/25
Flow rate    : 1.0mL/min
Detector     : UV(280nm)
Column temp. : 30deg-C

Courtesy of Dr. Oishi and Dr. Yamaki, Kitazato University