Shodex: Packed Columns for HPLC Analysis

HETEs (Hydroxyeicosatetraenoic Acids)

HETEs (hydroxyeicosatetraenoic acids) were analyzed using Asahipak ODP-50 6D ( a column for reversed phase chromatography ).


Sample : HETE
1. 15-HETE
2. 12-HETE
3. 5-HETE

Column       : Shodex Asahipak ODP-50 6D (6.0mmID*150mm)
Eluent       : H2O/CH3COOH/CH3CN/CH3OH=50/0.1/50/10
Flow rate    : 1.0mL/min
Detector     : UV(235nm)
Column temp. : 30deg-C

Courtesy of Dr. Oishi and Dr. Yamaki, Kitazato University