Analysis of Anions = Effect of pH on Retention Time (NI-424)

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Effect of pH on Retention Time (NI-424)

When using IC NI-424 and the pH of the eluent is increased on the alkaline side, overall elution shortens. At a pH of 4.5 or higher, separation of F^- and H₂PO₄^- is difficult.

Sample : 30micro-L
1. H₂PO₄^-
2. F^-
3. Cl^-
4. NO₃^-
5. SO₄^2-
6. I^-
7. SCN^-

Column       : Shodex IC NI-424 (4.6mmID*100mm)
Eluent       : ^*18mM 4-Hydroxybenzoic acid + 2.8mM Bis-Tris + 2mM Phenylboronic acid + 0.005mM ^*2CyDTA(pH4.2)
Flow rate    : 1.0mL/min
Detector     : Shodex CD
Column temp. : 40deg-C

^*1 The prescribed pH can be obtained by mixing the reagents in the composition described here.
^*2 CyDTA: trans-1,2-Diaminocyclohexane-N,N,N',N'-tetraacetic acid

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