Shodex: Packed Columns for HPLC Analysis

Total Cyanide in River Water

Total cyanide in river water was analysed using DEAE-825 (a column for weak anion exchange chromatography) and an electrochemical detector (ECD).


Sample : 100micro-L of distilled river water (water)

Column       : Shodex IEC DEAE-825 (8.0mmID*75mm)
Eluent       : (10mM Sodium carbonate + 1mM Ethylenediamine)/CH3OH=90/10
Flow rate    : 1.0mL/min
Detector     : ECD(Electrode; Ag, 0mV SCE)
Column temp. : 40deg-C