Shodex: Packed Columns for HPLC Analysis

5-(Hydroxymethyl)Furfural in Fruit Juice

5-(Hydroxymethyl)furfural (5-HMF) in fruit juice was analyzed using Asahipak ODP-50 6D (a column for reversed phase chromatography .



Sample : 5-(Hydroxymethyl)furfural, 5-HMF in Fruit juice
100% Natural orange juice
100% Natural grape juice

Column       : Shodex Asahipak ODP-50 6D (6.0mmID*150mm)
Eluent       : 50mM Sodium phosphate buffer(pH3.0)/CH3CN=90/10
Flow rate    : 1.0mL/min
Detector     : Shodex UV(283nm)
Column temp. : 50deg-C