Shodex: Packed Columns for HPLC Analysis

Cyanate and Carbamyl Phosphate

Cyanate and carbamyl phosphate were analyzed using Asahipak ES-502N 7C (a column for weak cation exchange chromatography).


Sample : 20micro-M each, 50micro-L
1. Carbamyl phosphate
2. Cyanate

Column       : Shodex Asahipak ES-502N 7C (7.6mmID*100mm)
Eluent       : 0.15M NaCl aq.
Reagent      : (I); 0.1M acetate buffer(pH3.0) containintg 2%(w/v) Phenol 
                    and 10mg/dL Sodium nitropreusside 
               (II); 1.0%(w/v) Sodium hydroxide containing 0.1%(w/v) Sodium hypochlorite
                (for hydrolysis under acidic condition/indophenol reaction)
Flow rate    : 1.0mL/min
Detector     : VIS(635nm)
Column temp. : 20deg-C

I.Koshiishi et al., Anal.Sci., 2 (1986) 81.