Shodex: Packed Columns for HPLC Analysis

Recovery of Various Drugs (PK-2A)

Recoveries of the various drugs by MSpak PK-2A ( a Column for Column switching ) were investigated. PK series can concentrate various drugs over the wide range of pH and hydrophobicity.



Sample : Drugs 2.5ppm each, 100micro-L

Pretreatment
Column         : Shodex MSpak PK-2A (2.0mmID*10mm)
Eluent         : 10mM CH3COONH4(pH7.0)
Flow rate      : 0.5mL/min
Column temp.   : Room temp.
Switching time : 5min
 
Analysis 
Column       : Shodex MSpak GF-4A (4.6mmID*10mm)
Eluent       : 10mM CH3COONH4(pH7.0)/CH3CN
Flow rate    : 0.5mL/min
Column temp. : Room temp.